29 Feb 2012 The accurate genotyping of human papillomavirus (HPV) is clinically important Luminex (xMAP) suspension array technology is based on

Vaccin mot HPV typ 6, 11, 16 och 18 finns tillgängligt, och kondylom av vilka kulor som är positiva med flödescytometri (Luminex-metod) [12].

METOD/ ANALYSPRINCIP Efter nukleinsyraextraktion av provmaterialet utförs PCR-amplifiering med hjälp av av HPV primers med förmåga att detektera ett brett spektrum av HPV hög- och lågrisk typer. PCR-produkten analyseras därefter så kallad Luminex-teknik för identifiering av HPV typ(er). Highly Sensitive Detection and Genotyping of HPV by PCR Multiplex and Luminex Technology in a Cohort of Colombian Women with Abnormal Cytology Dabeiba A García 1, Angel Cid-Arregui *, 2, Markus Schmitt 3, Marcos Castillo 4, 5, Ignacio Briceño 4, Fabio A. Aristizábal6 6 Kit 2: Multiplex High Risk HPV Real Time Fluorescent Detection. This kit can also detect all 15 high risk HPV genotypes in single tube by real time fluorescence detection.

Luminex hpv genotyping

Reproducibility testing found complete or partial concordance in 30/33 samples. 2020-10-23 · After amplification, the Luminex-based HPV genotyping allows the identification of the following HR-HPV types: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and the probable high-risk type 68 (A and B) as well as the possibly high-risk types 26, 53, 66, 67, 69, 73 and 82 as described by IARC classification from year 2012 . Genotyping of human papillomavirus in cervical lesions by L1 consensus PCR and the Luminex xMAP system. Jiang HL(1), Zhu HH, Zhou LF, Chen F, Chen Z. Author information: (1)Institute of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, People's Republic of China. 2017-01-03 · Luminex ® HPV genotyping of 37 HPV types HPV type-specific DNA probes covalently linked to fluorescence-labeled polystyrene beads (MAPx) were hybridized to HPV amplicons labeled with a spectrally distinct fluorophore and these hybrids were detected by two lasers that excite the two fluorophores in Luminex ® as described by Schmitt et al., [ 10 ]. Luminex‐based assays for HPV genotyping have proven to be highly sensitive, detecting just 1–10 template copies of plasmid per PCR reaction . Prior to clinical sample analysis, the two‐step PCR protocol was used to amplify positive control DNA sequences extracted from plasmids to validate the Cy5‐labelling strategy for the MPS assay.

The clinical evaluation was performed against the reference assay, BS-GP5+/6+ multiplex genotyping (MPG)-Luminex, with 600 cervical smear samples of a referral population. HPV DNA Array detected CIN2+ lesions with a sensitivity of 90.2%, identical to that of MPG-Luminex.

Journal of Medical Probe hybridization using xMAP® technology (Luminex Corporation) to identify 45 mucosal HPV types (6, 11, 13, 16, 18, 26, 30, 31, 32, 33, 35, 39, 40, 42, 43, 44, 9 Low-risk HPV genotypes : 6, 11, 40, 42, 43, 44, 54, 61, 70 Accurate genotyping of 28 HPV types in a single reaction b. In-house PCR Luminex.

The laboratory performs genotyping and comparative genomic studies of viruses, especially herpesviruses, and antiviral resistance for HSV and CMV. The laboratory carries out research and development studies on serological testing for VZV, and detection of polyomaviruses and HPV. It is now clear that HPV is the necessary cause of cervical cancer.

To improve HPV detection by PCR, the broad-spectrum L1-based SPF10 PCR DNA enzyme immunoassay (DEIA) LiPA system and a novel E6-based multiplex type-specific system (MPTS123) that uses Luminex xMAP technology were Luminex ® HPV genotyping of 37 HPV types HPV type-specific DNA probes covalently linked to fluorescence-labeled polystyrene beads (MAPx) were hybridized to HPV amplicons labeled with a spectrally distinct fluorophore and these hybrids were detected by two lasers that excite the two fluorophores in Luminex ® as described by Schmitt et al., [ 10 ]. The LMNX genotyping kit HPV GP (LMNX) is based on the clinically validated GP5/6 PCR, with a genotyping readout as an alternative for the more established enzyme immunoassay (EIA) detection of 14 targeted high-risk human papillomavirus (HPV) types. LMNX is additionally provided with an internal control probe. Nested PCR followed by genotyping using Luminex technology (1) (www.luminexcorp.com). The precise HPV type(s) are reported.

Cytometry Part A, 2011. Tiffany Blair. Qinghua Feng.
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Prior to clinical sample analysis, the two‐step PCR protocol was used to amplify positive control DNA sequences extracted from plasmids to validate the Cy5‐labelling strategy for the MPS assay.

HPV 16 on the Luminex xMAP system, also called the HPV DNA suspension array Apr 24, 2014 HPV genotyping. Multiplex PCR. Luminex.
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Kit 2: Multiplex High Risk HPV Real Time Fluorescent Detection. This kit can also detect all 15 high risk HPV genotypes in single tube by real time fluorescence detection. Comparing to Kit 1, it won’t offer any genotyping information for detected high risk HPV in the samples.

Seitenthema: "Borrelien Tests HPV Genotyping Kit - Luminex Technologie: About DiaMex". Erstellt von: Hauke-Heda Müller. Sprache: deutsch.